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Ursprungligen postat av
Robbejao
En fråga bara, vet du vad en SH-SY5Y cell är eller? För det värkar ju knappt forskarna veta då det vara finns väldig knapphändig info.
Saxat från wiki
SH-SY5Y cells show growth-related parameters that were typical or even superior to many described immortalized tumorderived cell lines. The proliferation capacity is stable over time and also beyond passage 10.[9]
SH-SY5Y has a dopamine-β-hydroxylase activity and can convert dopamine to norepinephrine. It will also form tumors in nude mice in around 3–4 weeks. The loss of neuronal characteristics has been described with increasing passage numbers. Therefore, it is recommended not to be used after passage 20 or verify specific characteristics such as noradrenaline uptake or neuronal tumor markers.
Så jadu. Det låter som än väldigt stabil cell på gott och ont.
Forskare använder den sedan 70-talet för att göra experiment på kemikalier och mediciner som påverkar dopamin receptorer.
Det är ändå anmärkningsvärt att de sett att 3-CMC börjat döda cellerna efter 48 H av höga doser. Detta skulle kunna förklara vara vissa som har missbrukat den blivit personlighetsförändrade, de har helt enkelt bränt sönder dopamin receptorerna.
SH-SY5Y is a human derived cell line used in scientific research. The original cell line, called SK-N-SH, from which it was subcloned was isolated from a bone marrow biopsy taken from a four-year-old female with neuroblastoma. SH-SY5Y cells are often used as in vitro models of neuronal function and differentiation. They are adrenergic in phenotype but also express dopaminergic markers and, as such, have been used to study Parkinson's disease, neurogenesis, and other characteristics of brain cells.
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